Amplification of complete insect mitochondrial genome in two easy pieces
1995
Roehrdanz, R.L.
The entire insect mitochondrial genome has been amplified in just two PCR reactions. 'Universal' insect primers and techniques to optimize amplification of long PCR products were used to obtain segments of the mitochondrial genomes of Helicoverpa zea and Heliothis virescens (Lepidoptera) ranging in size from 3.7 kb to 14 kb or about 25% to over 85% of a typical 16 kb mtDNA. Overlapping pairs of fragments contain the complete mtDNA sequence.
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书目信息
Insect molecular biology
卷
4
期
3
页码
169
- 172
ISSN
0962-1075
其它主题
Genome; Gene amplification; Mitochondrial; Insect; Genome
语言
英语
类型
Journal Article; Text
2024-02-28
MODS
