Isolation of bacteria at different points of Pleurotus ostreatus cultivation and their influence in mycelial growth

Pleurotus ostreatus is one of the most cultivated edible mushrooms worldwide and few approaches have been done to analyze bacterial influence during its cultivation. Therefore, bacteria from commercial spawn, mycelial-colonized straw and fruiting bodies from healthy productive samples were counted, isolated and tested for their mycelial growth promoting ability. Bacterial cell numbers at different steps of the process showed low bacterial cell numbers in spawn and in fruiting bodies inner tissue compared to the high concentration in mycelial-colonized straw. The majority of the 38 isolates belonged to phyla Firmicutes and Actinobacteria were identified as Bacillus, Paenibacillus and Micromonospora species. Similarly, 16S rRNA gene bacterial clones obtained from mycelial biomass DNA samples showed bacterial presence of various genera including Bacillus and Paenibacillus.In the mycelial growth promoting ability tests, 30 isolates negatively affected mycelial growth, two isolates showed no effect on mycelial growth, and six isolates promoted mycelial growth. Moreover, mycelial thickness was influenced in different ways by the bacterial growth. In general, nearly all isolates growth-preventing were isolated from healthy spawn and mycelial-colonized straw, whereas fruiting bodies were the best source for isolation of mycelial growth-promoting bacteria. Characterization of bacterial isolates revealed that growth-preventing isolates exhibited various enzymatic activities in comparison with positive influencing bacteria that exhibited none or weak enzymatic activities. In addition, the influence of volatile compounds being present in the headspace of bi-plate co-cultures on P. ostreatus mycelial growth was demonstrated.The effect of isolates, that promoted mycelial growth in co-cultivation, to reduce P. ostreatus spawn running time, was evaluated on sterilized rye seeds. Results showed that not all mycelial promoted isolates were able to significantly promote P. ostreatus colonization. However, isolate M46F identified as Micromonospora lupini significantly reduce spawn running time. This is one of few studies to estimate cultivable bacteria from healthy samples of P. ostreatus cultivation, to evaluate a bacterial effect on mycelial growth, to show that fruiting bodies are a good source for mycelia growth-promoting isolates, and the first to report a shorter P. ostreatus spawn running time due to bacterial inoculation.