Genetic diversity of almonds (Prunus dulcis) using RAPD technique
2003
MirAli, N. | Nabulsi, I.
Randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships between 19 almond cultivars grown at two gene banks in southern Syria (Izraa and Jillin). Forty decamer primers were used and only one (OP-I14) did not produce any polymorphism. The remaining 39 primers ranged in their amplification fragments between one (OP-I19 and OP-N20) and eight (OP-A20, OP-N14, OP-N16, OP-R16, and OP-Z17). The generated similarity matrix showed that the genetic diversity within the tested genotypes was limited (average similarity index=0.78). Similarity values among the studied genotypes ranged between 0.70 and 0.96. The resulting dendogram divided the cultivars into two clusters (at 0.77 similarity value) with two cultivars (ACSAD12 and Ferralise) as the most distant from the group (similarity value 0.75). Mantel Test Statistic showed that the minimum number of primers needed to discriminate among almond cultivars studied was using either 20 random primers or the 14 most polymorphic fragments (six or more polymorphisms/primer) primers. The obtained clustering based on RAPD markers agreed to some extent with the geographical origin of the studied set of almond cultivars.
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