Intratracheal exposure of common marmosets to MERS-CoV Jordan-n3/2012 or MERS-CoV EMC/2012 isolates does not result in lethal disease
2015
Johnson, Reed F. | Via, Laura E. | Kumar, Mia R. | Cornish, Joseph P. | Yellayi, Srikanth | Huzella, Louis | Postnikova, Elena | Oberlander, Nicholas | Bartos, Christopher | Ork, Britini L. | Mazur, Steven | Allan, Cindy | Holbrook, Michael R. | Solomon, Jeffrey | Johnson, Joshua C. | Pickel, James | Hensley, Lisa E. | Jahrling, Peter B.
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) continues to be a threat to human health in the Middle East. Development of countermeasures is ongoing; however, an animal model that faithfully recapitulates human disease has yet to be defined. A recent study indicated that inoculation of common marmosets resulted in inconsistent lethality. Based on these data we sought to compare two isolates of MERS-CoV. We followed disease progression in common marmosets after intratracheal exposure with: MERS-CoV-EMC/2012, MERS-CoV-Jordan-n3/2012, media, or inactivated virus. Our data suggest that common marmosets developed a mild to moderate non-lethal respiratory disease, which was quantifiable by computed tomography (CT), with limited other clinical signs. Based on CT data, clinical data, and virological data, MERS-CoV inoculation of common marmosets results in mild to moderate clinical signs of disease that are likely due to manipulations of the marmoset rather than as a result of robust viral replication.
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