Micropropagation of Rosa pomifera
2014
Sedlak, J. | Paprstein, F.
The purpose of this study was to develop an efficient micropropagation system for edible cultivars of apple rose (Rosa pomifera). Two genotypes, 'Karpatia' and 'Belecska', were successfully established in vitro using mercuric chloride or sodium hypochlorite as sterilization solutions. In total only 19.2% of the initial explants of both genotypes were found to be contaminated with micro-organisms, all from explants treated with sodium hypochlorite. Six MS-based media containing 1, 2 or 4 mg L-1 BAP (6-benzylaminopurine), 0.5 or 1 mg L-1 TDZ (thidiazuron) or 10 mg.L-1 2iP (6-(γ,γ-dimethylallylamino) purine) were tested for initiation of multiplication. Generally, the highest proliferation rate (5.1) was obtained for 'Belecska' on MS medium with the highest concentration of BAP (4 mg L-1). The lowest shoot number (1.1) was noted for cultivar 'Karpatia' on MS medium with 1 mg L-1 TDZ. Abundant callus formation at the base of explants and abnormally narrow undeveloped leaves were observed on the media containing TDZ with 'Karpatia'. The cytokinin 2iP at 10 mg L-1 did not promote satisfactory proliferation in the two apple rose genotypes tested in our experiments. Rooting was achieved in both genotypes tested, but the root induction was relatively low from 17 to 57% of in vitro rooted plants. Generally, the best rooting (57%) was obtained with cultivar 'Belecska'on medium supplemented with 1 mg L-1 NAA. On the contrary, cultivar 'Karpatia' had the lowest rooting percentage 17% on medium with 1 mg L-1 IAA.
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