γ-Glutamyl-S-allyl-cysteine inhibits hepatic stellate cell proliferation and collagen secretion via a proapoptotic mechanism
2015
Zhang, Bimi | Tan, Dehong | Bai, Bing | He, Yin | Ji, Shujuan
We investigated the effects of γ-glutamyl-S-allyl-cysteine (GSAC) on the activated HSC cell line HSC-T6. GSAC inhibited the proliferation of HSC-T6 cells for 24 h with a half inhibitory concentration of 0.084 ± 0.005 mg/mL. FITC–Annexin V/PI double staining revealed that concentrations of 0.02, 0.04, and 0.08 mg/mL GSAC induced apoptosis at rates of 4.72 ± 0.83, 8.56 ± 1.94, and 18.63 ± 0.98 %, respectively. A DNA ladder pattern was observed in cells treated with 0.04 and 0.08 mg/mL GSAC in agarose gel electrophoresis of genomic DNA. The mitochondrial membrane potential decreased and the intracellular reactive oxygen species (ROS) increased in a concentration-dependent manner in GSAC-treated cells. Western blot analyses revealed that GSAC down-regulated TGF-β1, collagen I, and Bcl-2 and up-regulated Bax. GSAC also significantly increased the activities of caspase-3 and caspase-9. These results indicate that GSAC inhibited the proliferation and collagen secretion of HSC-T6 cells and that induction of ROS and apoptosis through a mitochondrial pathway may play important roles in the underlying mechanism.
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