Clostridium culturomicium sp. nov. and Clostridium jeddahitimonense sp. nov., novel members of the Clostridium genus isolated from the stool of an obese Saudi Arabian
2021
Kieu, Hoang Thong | Garrigou, Noémie | Fadlane, Amael | Brechard, Ludivine | Armstrong, Nicholas | Decloquement, Philippe | Yāsir, Muḥammad | Azhar, Esam Ibraheem | Al-Masaudi, Saad B. | Lagier, Jean-Christophe | Tidjani Alou, Maryam | Raoult, Didier
Taxono-genomics is an innovative concept coined for the description of new bacterial species. Phenotypic characteristics were combined with a genomic approach to describe two new species within the Clostridium senso stricto genus: Clostridium culturomicium strain CL-6ᵀ and Clostridium jeddahitimonense strain CL-2ᵀ, both isolated from the gut microbiota of an obese man from Saudi Arabia. Strains CL-6ᵀ and CL-2ᵀ shared a similarity of 98.4% with the 16S rRNA gene of Clostridium subterminale strain JCM 1417ᵀ (accession number NR113027) and 98% with that of Clostridium disporicum strain DS1ᵀ (accession number NR026491), respectively. The highest OrthoANI values were shared with Clostridium punense for strain CL-6ᵀ (70.8%) and with Clostridium disporicum for strain CL-2ᵀ (87.1%). Additionally, strain CL-6ᵀ and strain CL-2ᵀ shared a 16S rRNA similarity of 91.4%. Both strains were anaerobic, spore-forming and Gram-stain-positive non-motile bacilli. The genome of Clostridium culturomicium strain CL-6ᵀ is 4,325,182 bp long with 32.2% GC content. As for Clostridium jeddahitimonense strain CL-2ᵀ, the genome is 4,074,758 bp long with 29.2% GC content.
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