T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis
2016
Guo, Wei | Sun, Shuhui | Xie, Binghua | Zhu, Xiao-Jing | Dai, Zhong-Min
Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM). However, MSDM is sometimes difficult. Here we demonstrated that T4 DNA polymerase greatly enhanced the efficiency of MSDM. Moreover, we have also showed that it is efficient to clone multiple specific mutation-containing sequences simultaneously.
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书目信息
出版者
Taylor & Francis
其它主题
Multiple site-directed mutagenesis; Dna-directed dna polymerase; T4 dna polymerase; Site-directed mutagenesis; Complementary annealing mediated by exonuclease; Dna assembly
语言
英语
注释
Nal-ap-2-clean
This work was supported by the Natural Science Foundation of Zhejiang Province [grant number LY15C090008], [grant number LY16C120002].
类型
Journal Article; Text
2024-02-29
2026-02-03
MODS