Effect of different lectins on the proliferation of goat peripheral blood mononuclear cells
2002
Regis, L. | Ribeiro, M. | Vale, V. | Paule, B. | Nascimento, I. | Schaer, R. | Meyer, R. | Freire, S.
In the present study we determined the effect of the lectins concanavalin A (Con A), phytohemagglutinin (PHA) and pokeweed (PWM), frequently used in cell proliferation assays, on the proliferation of goat peripheral blood mononuclear cells (PBMCs). The use of a standardized Percoll gradient proved to be highly effective for the separation/enrichment of these cells, without damaging the cell membrane. Pokeweed and phytohemagglutinin showed a high stimulating effect, with proliferation rates of 16,753 and 2350 counts/min (cpm), respectively, whereas Con A showed very low stimulation indices (495 cpm), supporting the notion that it should not be used for blast proliferation studies in goats.
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