The effects of calcium deficiency on Cucurbita pepo L. hypocotyl cells: a 31P nuclear-magnetic-resonance study
1993
Allan, A.C. | Shachar-Hill, Y. | Rubery, P.H.
Calcium deficiency in zucchini (Cucurbita pepo L.) is associated with reduced growth and a reduced ability to transport auxin (Allan and Rubery, 1991, Planta 183, 604-612). An investigation of the effects of calcium-deficiency on zucchini hypocotyl cells was made using weak-acid uptake and 31P-nuclear-magnetic-resonance (31P-NMR) spectroscopy in vivo and in tissue extracts. Calcium-deficient tissue had the same cytoplasmic and vacuolar pHs as normal tissue when extracellular pH was near neutral. At acidic external pH the vacuolar pH was lower in deficient tissue. Adenine nucleotides were present predominantly as ATP in both control and calcium-deficient tissues. Addition of calcium to calcium-deficient tissue, under conditions which cause recovery of auxin transport induced no changes in the 31P-NMR spectra of deficient tissue. The content of mobile, phosphorylated metabolites was reduced in calcium-deficient tissue in comparison to control tissue. However, a substantial increase in the content of phosphorylcholine occurs in calcium-deficient tissues compared with controls; this may reflect changes in lipid turnover in calcium-stressed cells.
显示更多 [+] 显示较少 [-]