Regulatory effects and anti‐inflammatory activity of Trachyspermum ammi (L.) Sprague seeds extract on alleviation of kidney injury in diabetic rats
2022
Zolfaghari, Najimeh | Monajemi, Ramesh | ShahaniPour, Kahin | Ahadi, Ali Mohammad
Diabetic nephropathy is a major cause of renal tissue damage and complications of diabetes. In this in‐vitro experimental study, diabetes was induced by a single injection of Streptozotocin (55 mg/kg) in rats. STZ‐diabetic rats were treated with oral doses of methanolic extract T. ammi seeds (300–400 mg/kg). After 8 weeks, serum levels of glucose, creatinine, urea, SOD, and MDA in the blood serum and protein levels in urine were measured. Also, changes in the expression of IL‐18 and TNF‐α genes in the renal tissue were examined. The results of this study showed that T. ammi seeds have anti‐hyperglycemic and anti‐hyperlipidemia effects. It can also increase SOD, decrease MDA, and inhibit IL‐18 and TNF‐α expression. T. ammi methanolic extract reduced blood glucose, oxidative stress, renal function biomarkers, and inflammatory cytokines caused by STZ in mice. Because of this, it could be used as a new treatment for diabetic nephropathy. NOVELTY IMPACT STATEMENT: The effect of T. ammi seed extract on rats with nephropathy was investigated. The real‐time PCR technique was used to measure the expression of the genes IL‐18 and TNF‐a, while the ELISA technique was used to measure the level of MDA and SOD serum. T. ammi is a medicinal plant with anti‐inflammatory and anti‐nephropathy effects.
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