Detection of fungal spores from contaminated surfaces by the polymerase chain reaction
1999
Hernández, Alicia | Martínez, José L. | Mellado, Rafael P.
A method has been developed to detect fungal spores in dust samples collected from internal surfaces of air-conditioning ducts. The method is based on the utilization of the polymerase chain reaction (PCR) with specific primers for fungal species. PCR amplification is carried out directly in boiled samples avoiding time-consuming DNA preparation steps. The presence of bovine serum albumin in the reaction mixture overcame the inhibitory effect of the humic acids present in the dust.
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书目信息
出版者
Kluwer Academic Publishers
其它主题
Bovine serum albumin
语言
英语
类型
Journal Article; Text
2024-02-29
MODS