The effects of alkythioacetic acids (3-thia fatty acids) on fatty acid metabolism in isolated hepatocytes
1989
Skrede, S. | Narce, M. | Bergseth, S. | Bremer, J.
Long-chain alkylthioacetic acids (3-thia fatty acids) inhibit fatty acid synthesis from [1-(14)C]acetate in isolated hepatocytes, while fatty acid oxidation is nearly unaffected or even stimulated. Desaturation of [1-(14)C]stearate (delta (9)-desaturase) is also unaffected. [1-(14)C]Dodecylthioacetic acid (a 3-thia fatty acid) is incorporated in triacylglycerol and in phospholipids more efficiently than [1-(14)C]palmitate in isolated hepatocytes. The metabolism of [1-(14)C]dodecylthioacetic acid to acid-soluble products (by omega-oxidation) is slow compared to the oxidation of [1-(14)C]palmitate. In hepatocytes from adapted rats (rats fed tetradecylthioacetic acid for 4 days) the rate of [1-(14)C]palmitate oxidation is increased and its rate of esterification is decreased. Stearate desaturation is also decreased. The rate of cyanide-insensitive peroxisomal fatty acid beta-oxidation is several-fold increased. The metabolic effects of long-chain 3-thia fatty acids are discussed and it is concluded that they behave essentially like normal fatty acids except for their slow breakdown due to the sulfur atom in the 3 position, which blocks normal beta-oxidation.
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