Sensitivity of sublethally injured gram-negative bacteria to pediocin P
2005
Osmanagaoglu, O.
All Gram-negative bacteria tested were normally resistant to the bactericidal action of pediocin P of Pediococcus pentosaceous Pep1, which had been isolated from vacuum-packaged sausages. However, when they were subjected to sublethal stress (exposure to physical and chemical stresses such as freezing, heating and acid treatment) and were treated with pediocin P, gram-negative bacterial cells that were normally resistant to pediocin P developed sensitivity to it and pediocin P reduced the viability of bacterial cells surviving sublethal stresses, although the extent of reduction varied with strain and type of treatment. When the combined effect of ethylenediaminetetraacetic acid (EDTA), sublethal heat and pediocin P was studied by adding EDTA and pediocin P to cell suspensions right before heating them, there was a considerable reduction in the number of viable cells, even at the lowest concentration of pediocin P tested. The results of our preliminary studies showed that sublethal injury can make the gram-negative bacterial cells sensitive to pediocin P.
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