Production of podophyllotoxin in Juniperus chinensis callus cultures treated with oligosaccharides and a biogenetic precursor
1998
Muranaka, T. | Miyata, M. | Ito, K. | Tachibana, S.
Calli were induced from the leaves of young trees of Juniperus chinensis on Schenk and Hildebrandt medium supplemented with napthalenacetic acid and kinetin and subcultured on the same medium. Podophyllotoxin, a strong anti-tumor agent, was isolated from the extractives of calli and found that calli produced podophyllotoxin. The podophyllotoxin in the calli derived from the leaves constituted 0.005% of dry weight. In contrast, the content of podophyllotoxin in intact plant was 0.0025% of dry weight. The cultures of the calli produced twice as much podophyllotoxin as did those of the intact plant. The production of podophyllotoxin was increased fifteen-fold by addition of chito-oligosaccharides, an elicitor, to the calli. And furthermore, the production increased eleven-fold by addition of phenylalanine, a biogenetic precursor of podophyllotoxin.
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