Characterization of monoclonal antibodies to hemocytes of Pseudoplusia includens
1996
Strand, M.R. | Johnson, J.A.
A panel of monoclonal antibodies (MAbs) was generated against hemocytes from unparasitized Pseudoplusia includens and P. includens parasitized by the polydnavirus-carrying parasitoid Microplitis demolitor. Of 862 hybridomas generated, 107 lines secreted antibodies to one or more classes of hemocytes. For the current study, 21 lines were cloned, 11 lines were isotyped and three MAbs were characterized. MAb 48F2D5 specifically labeled granular cells, MAb 52F3A5 labeled plasmatocytes and MAb 55F2G7 only labeled hemocytes inoculated with M. demolitor polydnavirus (MdPDV). Western blot analysis indicated that each MAb recognized specific antigens when cell proteins were separated on SDS-PAGE gels. Using immunofluorescence microscopy, differences were noted in the proportion of granular cells and plasmatocytes present in parasitized hosts and in the proportion of each cell type labeled by MAb 55F2G7. Double-labeling experiments further suggested that the proportion of granular cells infected by MdPDV declined between 24 h and 7 days post-parasitism while the proportion of infected plasmatocytes did not.
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