Circulating miR-21 is Down-Regulated Following Adjuvant Chemotherapy in Breast Cancer Patients
2020
Kazemi Nezhad, S. R. | Baghbani, H. | Sasan, H. | Hajjari, M. R.
Breast cancer is one of the most prevalent known cancers in women with the incidence rate of annually 7000 cases in Iran. MicroRNAs (miRNAs) are a group of non-coding RNAs with 20–25 nucleotides length, which can effect on cancer development. There is low information about the influence of anti-cancer drugs on the expression levels of miRNAs. The present study is the first investigation to evaluate the down-regulation of circulating microRNA-21 (miR-21) expression in breast cancer patients after treatment with Doxorubicin and 5-fu drugs. The expression levels of miR-21 in paired blood plasma samples of 40 new cases of breast cancer patients, were analyzed by Real-Time Polymerase Chain Reaction (RT-PCR) with 2 repeats for each sample. miR-21 rates were evaluated before and after the treatment with Doxorubicin and 5-FU medicines and data were analyzed using t-test. Comparisons between plasma miRNAs before and after the chemotherapy suggested that the treatment has down regulated the circulating miR-21 expression in both HER2+ (Human Epidermal growth factor Receptor 2 positive) and HER2—breast cancer patients. According to the obtained results, it can be suggested that Doxorubicin and 5-FU drugs significantly decrease the circulating miR-21 expression, which might play a key role in anti-tumor effects (0.15 Fold change, P-value < 0.0001).
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