A transcriptional roadmap to wood formation
2001
Hertzberg, M. | Aspeborg, H. | Schrader, J. | Andersson, A. | Erlandsson, R. | Blomqvist, K. | Bhalerao, R. | Uhlen, M. | Teeri, T.T. | Lundeberg, J.
The large vascular meristem of poplar trees with its highly organized secondary xylem enables the boundaries between different developmental zones to be easily distinguished. This property of wood-forming tissues allowed us to determine a unique tissue-specific transcript profile for a well defined developmental gradient. RNA was prepared from different developmental stages of xylogenesis for DNA microarray analysis by using a hybrid aspen unigene set consisting of 2,995 expressed sequence tags. The analysis revealed that the genes encoding lignin and cellulose biosynthetic enzymes, as well as a number of transcription factors and other potential regulators of xylogenesis, are under strict developmental stage-specific transcriptional regulation.
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