Phylogenetic and selection pressure analyses of cold stress-associated PAL-Like and Lec-RLK genes in antarctic mosses
2020
Minozzo, Mônica Munareto | Metz, Geferson Fernando | de Vargas, Maria Victória Magalhães | Pereira, Antônio Batista | Victoria, Filipe de Carvalho
Over time plants undergone abiotic changes often leading to genotypic and phenotypic level adjustments to ensure survival. Temperature is one of these factors that cause changes. Several organisms have been sequenced in recent years, creating ways to different direction biological and evolutionary issues. It has been allowed the use of these non-model biological systems for re-sequencing of close relatives, or the same species, as well as allowing the mapping and identification of gene families and genes of interest. For this work were focused on obtaining data from mosses available in the Sequence Reads Archive (SRA): Physcomitrium acutifolium (SRP093878); Funaria hygrometrica (ERP002608); Ceratodon purpureus (SRP004754); Pohlia nutans (SRP012293); Bryum argenteum (SRX845477). The data obtained were compared in bioinformatical environment in order to evaluate the GO terms in each plant transcriptome.The number of transcripts was expressed in a very divergent way among the organisms, with F. hygrometrica counting in value (over 100,000), while P. acutifolium in the same family had the lowest number of transcripts (∼ 27,000). Comparing the occurrences transcripts related to abiotic stress from the species used, were found GO terms for phosphorolization proteins coding genes expressed, such protein kinase-like activity, and transcriptions factor activities, as Lectin domain of the RLK group, as the most frequent ontology in all moss transcriptomes. Our findings allow us to direct the researches in order to understand how these regions act effectively in the molecular response to cold stress in plants, contributing to the understanding of this process during the evolution of green plants. Consequently, the findings may contribute to strategies for the assisted improvement of plants of agronomic and industrial importance.
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