Chlorophyll production from Spirulina platensis: cultivation with urea addition by fed-batch process
2004
Rangel-Yagui, C. de O. | Danesi, E.D.G. | Carvalho, J.C.M de | Sato, S.
The cyanobacterium Spirulina platensis is an attractive alternative source of the pigment chlorophyll, which is used as a natural color in food, cosmetic, and pharmaceutical products. In this work, the influence of the light intensity and urea supplementation as a nitrogen source using fed-batch cultivation for S. platensis growth and chlorophyll content was examined. Cultivations were carried out in 5 l open tanks, at 30 +/- 1 degrees C. Response surface methodology was utilized for analysis of the results, and models were obtained for biomass productivity, nitrogen-cell conversion factor and chlorophyll productivity. The best cellular growth was observed with 500 mg/l of urea at a light intensity of 5600 lx, whereas the highest concentration of chlorophyll in the biomass was observed with 500 mg/l of urea at a light intensity of 1400 lx. Overall, the best chlorophyll productivity was observed with 500 mg/l of urea at a light intensity of 3500 lx, providing the optimal balance between the cellular growth and the biomass chlorophyll content.
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