Identification of atypical Frankia strains by fatty acid analysis
1991
Mirza, M.S. | Janse, J.D. | Hahn, D. | Akkermans, A.D.L.
Ineffective, non-infective actinomycetous isolates obtained from actinorhizal nodules of Coriaria nepalensis and Datisca cannabina were identified as Frankia using whole cell fatty acid analysis. The isolates exhibited fatty-acid patterns very similar to those of confirmed Frankia strains from other host plants (Alnus, Casuarina, Colletia, Comptonia, Elaeagnus and Hippophae). All Frankia strains, including Coriaria and Datisca isolates, showed fatty-acid profiles very distinct from those of other actinomycetes used as controls (Actinomyces, Geodermatophilus, Nocardia, Mycobacterium and Streptomyces). For the genus Frankia, a characteristic pattern of five fatty acids (15:0; 15:1; 16:0 iso;17:0 and 17:1) was found. These fatty acids comprised 75% or more of the total content. All Frankia strains could be placed into three subgroups. Coriaria isolates were found in the largest subgroup which contained most Frankia strains from other hosts while ineffective strains from Alnus, Elaeagnus and Datisca were distributed in all three subgroups of Frankia.
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