In vitro antiviral activity from Acanthospermum australe on herpesvirus and poliovirus
2011
Martins, Lucia Regina Rocha | Brenzan, Mislaine Adriana | Nakamura, Celso Vataru | Dias Filho, Benedito Prado | Nakamura, Tania Ueda | Cortez, Lucia Elaine Ranieri | Cortez, Diogenes Aparicio Garcia
Context: The Asteraceae family has been of interest to researchers due to the presence of polyphenolic compounds, mainly flavonoids, which demonstrated antiviral activity. Objective: The hydroethanol extract of the aerial parts of Acanthospermum australe (Loefl.) Kuntze (Asteraceae) and its fractions, were evaluated in vitro for their potential cytotoxic and antiviral activity against bovine herpesvirus and human poliovirus. Materials and methods: The sulforhodamine B colorimetric assay were used to evaluate the capacity of the hydroethanol extract and fractions to inhibit the lytic activity of herpes and poliovirus in infected cell cultures and their influence on the viability of uninfected cell cultures. Results and discussion: A progressive increase in the antiviral effect against herpesvirus was observed in the course of the purification process of the extract. The hydroethanol extract had a 50% antiviral effective concentration (EC50) at 70 μg/mL and 36 μg/mL for herpes and poliovirus, respectively, and it exhibited no cytotoxicity. The fractions F3 (dichloromethane) and F4 (dichloromethane: ethyl acetate (1:1 v/v)) both showed EC50 at 6.25 μg/mL against herpesvirus, and these fractions showed cytotoxic concentrations (CC50) at 12.7 and 11.7 μg/mL, respectively. These fractions had no effect against poliovirus in the concentrations tested. From the bioactive F3, a diterpene lactone (acanthoaustralide-1-O-acetate) was isolated at a concentration of 0.5% and from F4 two flavonoids (quercetin and chrysosplenol D) were isolated at concentrations of 0.14 and 0.24%, respectively. Conclusion: The present study reports for the first time the antiviral activity of extracts and fractions from A. australe aerial parts.
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