Citrus tristeza virus: characterization of coat proteins
1988
Lee, R.F. | Calvert, L.A. | Nagel, J. | Hubbard, J.D.
The coat proteins of several aphid-transmitted Florida isolates of citrus tristeza virus (CTV) were characterized by polyacrylamide gel electrophoresis, western blotting followed by serological probing, and polypeptide mapping on sodium dodecyl sulfate polyacrylamide gels following treatment with proteolytic enzymes. All isolates had a major coat protein (CP1) of about Mr 23,000 and a minor coat protein (CP2) of about Mr 21,000, both of which reacted with CTV-specific antisera when using western blot and enzyme-linked immunosorbent assays. The amino acid composition of CPI was similar to that reported for the coat proteins of other closteroviruses. Polypeptide maps of CP1 and CP2 were similar. In vitro translation of RNA extracted from purified virions produced proteins of various sizes, but only one peptide of about Mr 26,000 was immunoprecipitated with CTV-specific antiserum.
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