Detection and discrimination of nutrient deficiencies in sunflower by blue-green and chlorophyll-a fluorescence imaging
2011
Cadet, Émilie | Samson, Guy
Fluorescence imaging was utilized to demonstrate the potential of blue-green fluorescence (BGF) and chlorophyll-a fluorescence (ChlF) to discriminate nitrogen (N), phosphorus (P), and potassium (K) deficiencies in sunflower plant showing similar growth inhibition. Only K-deficient leaves displayed significant increase of the BGF intensity. The epidermal UV-transmittance estimated by the ratio of ChlF intensities induced by UV and blue excitations (ChlFUV/ChlFBLUE) markedly decreased in both N- and P-deficient leaves but only in the latter that we observed significant decrease of the ratio of red and far-red ChlF intensities (RF/FRF) (that is inversely related to leaf chlorophyll concentration). The BGF increase in K-deficient was limited at leaf apex and margins and was spatially correlated to localized RF/FRF increases. Images analysis allows a better interpretation of the fluorescence changes by showing the spatial relationships between BGF, the ChlFUV/ChlFBLUE and the RF/FRF ratios that are indicative of physiological disturbances occurring in leaves of nutrient deficient plants.
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