Identification of a novel metabolite in phenanthrene metabolism by the fungus Cunninghamella elegans
1989
Cerniglia, C.E. | Campbell, W.L. | Freeman, J.P. | Evans, F.E.
The metabolism of phenanthrene by the fungus Cunninghamella elegans was investigated. Kinetic experiments using [9-(14)C]phenanthrene showed that after 72 h, 53% of the total radioactivity was associated with a glucoside conjugate of 1-hydroxphenanthrene (phenanthrene 1-O-beta-glucose). This metabolite was isolated by reversed-phase high-performance liquid chromatography and characterized by the application of UV absorption, 1H nuclear magnetic resonance, and mass spectral techniques. The results show that aromatic ring oxidation followed by glucosylation is a predominant pathway in the metabolism of the polycyclic aromatic hydrocarbon phenanthrene by C. elegans.
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