Aliiglaciecola coringensis sp. nov., isolated from a water sample collected from mangrove forest in Coringa, Andhra Pradesh, India
2014
Gupta, Vasundhera | Sharma, Gunjan | Srinivas, T. N. R. | Anil Kumar, P.
A Gram-negative, rod shaped, motile, aerobic bacterium, designated as strain AK49ᵀ was isolated from a water sample from a mangrove forest in Coringa village, Andhra Pradesh, India. Strain AK49ᵀ was observed to form yellow coloured, smooth, circular, convex colonies on marine agar, with entire margins. Cells of strain AK49ᵀ are 0.5–1.0 µm wide and 1.5–3.5 µm long. Growth was observed at 25–37 °C (optimum 30 °C), 2–6 % NaCl (optimum 2 %) and pH 6–8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain AK49ᵀ is closely related to two species recently reclassified as members of the genus Aliiglaciecola: Aliiglaciecola lipolytica JCM 15139ᵀ (sequence similarity 95.43 %) and Aliiglaciecola litoralis JCM 15896ᵀ (sequence similarity 96.91 %). The major cellular fatty acids of strain AK49ᵀ were found to include C₁₆:₀, C₁₈:₁ω7c and summed feature 3 (C₁₆:₁ω7c/C₁₅:₀ iso-2-OH). The polar lipid content of cell membrane was found to include phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified lipid and an unidentified glycolipid. The genomic DNA G+C content of strain AK49ᵀ was determined to be 41.9 mol%. Based on the taxonomic methods, including chemotaxonomic, phenotypic and phylogenetic approaches, strain AK49ᵀ is described here as a novel species belonging to the genus Aliiglaciecola, for which the name Aliiglaciecola coringensis sp. nov. is proposed. The type strain of Aliiglaciecola coringensis sp. nov. is AK49ᵀ (=MTCC 12003ᵀ = JCM19197ᵀ).
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