Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana
2015
Mork-Jansson, Astrid Elisabeth | Gargano, Daniela | Kmiec, Karol | Furnes, Clemens | Shevela, Dmitriy | Eichacker, Lutz Andreas
The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 from Arabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25±7.8nM) is favored relative to homodimerization (431±59nM). Interaction of Lil3.2 with chlorophyll a (231±49nM) suggests that heterodimerization precedes binding of chlorophyll in Arabidopsis thaliana.
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