Induction of Xylanase from Trichoderma viride by using Different Carbon Sources
2012
Shahid, Mohd | Singh, Anuradha | Srivastava, Mukesh | Rastogi, Smita | Pathak, Neelam
Trichoderma viride strain 01PP produced extra cellular xylanases when grown in liquid medium cultures containing oat spelt xylan and birch wood xylan as inducer. Trichoderma viride 01PP was grown in Vogel's medium with different carbon sources such as glucose, maltose, carboxymethylcellulose (CMC), sucrose, corn cobs, wheat bran, oat spelt xylan and birch wood xylan at 28ºC with shaking at 150 rpm to check the induction of xylanase. Expression of xylanase was induced maximally by xylan and repressed by glucose. The pH and temperature optima of the crude xylanase were 5.5 and 28ºC, respectively. Highest xylanase activity (5.5±0.01 IU/mL) was observed at 120 hours with 1% birch wood xylan as a carbon source. T. viride did not show xylanolytic activity without the presence of any carbon source.
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