Characterization of a hydrogen-producing granular sludge
2002
Fang, H.H.P. | Liu, H. | Zhang, T.
This study demonstrated that hydrogen-producing acidogenic sludge could agglutinate into granules in a well-mixed reactor treating a synthetic sucrose-containing wastewater at 26 degrees C, pH 5.5, with 6 h of hydraulic retention. A typical matured granule is 1.6 mm in diameter, 1.038 g/ml in density, 11% in ash content, and over 50 m/h in settling velocity. Treating a solution containing 12.15 g/L of sucrose at a volumetric loading rate of 48.6 g/L(.)d), the reactor containing 20 g/L of granular sludge degraded 97% of sucrose. Effluent comprised 46% acetate and 49% butyrate and the methane-free badges comprised 63% hydrogen, 35% carbon dioxide, and 2% nitrogen. Hydrogen production rate was 13.0 L/(L(.)d), and the yield was 0.28 L/g-sucrose. The granule had multiple cracks on the surface and comprised two morphological types of bacteria: fusiform bacilli and a spore-forming bacterium. Phylogenetic analysis showed that 69.1% of the clones were affiliated with four Clostridium species in the family Clostridiaceae, and 13.5% with Sporolactobacillus racemicus in the Bacillus/Staphylococcus group.
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