Antifouling assessments on biogenic nanoparticles: A field study from polluted offshore platform
2015
Krishnan, Muthukumar | Sivanandham, Vignesh | Hans-Uwe, Dahms | Murugaiah, Santhosh Gokul | Seeni, Palanichamy | Gopalan, Subramanian | Rathinam, Arthur James
Turbinaria ornata mediated silver nanoparticles (TOAg-NPs) were evaluated for antibacterial activity against 15 biofilm forming bacterial isolates. A field study in natural seawater for 60days showed antifouling activity of TOAg-NPs on stainless steel coupons (SS-304) coated with Apcomin zinc chrome (AZC) primer. Though TOAg-NPs showed broad spectrum of antibacterial activity, the maximum zone of inhibition was with Escherichia coli (71.9%) and a minimum with Micrococcus sp. (40%) due to the EPS secretion from Gram-positive bacteria. Compared to control coupons (18.9 [×103], 67.0 [×103], 13.5 [×104] and 24.7 [×104]CFU/cm2), experimental biocide coupons (71.0 [×102], 32.0 [×103], 82.0 [×103] and 11.3 [×104]CFU/cm2) displayed lesser bacterial population density. Toxicity studies revealed 100% mortality for Balanus amphitrite larvae at 250μgml−1 concentration within 24h, while 56.6% recorded for Artemia marina at the same concentration indicating less toxicity to non target species. It proved that AZC+TOAg-NPs prevent biofouling by its Ag-NS affinity and antimicrobial effectivity.
显示更多 [+] 显示较少 [-]