Somatic embryogenesis and long term maintenance of embryogenic lines from fox grapes
2001
Motoike, S.Y. | Skirvin, R.M. | Norton, M.A. | Otterbacher, A.G.
In the present paper, a method for the induction and long-term maintenance embryogenic cultures for Vitis x Labruscana 'Niagara' and 'Fredonia' is reported. Embryogenic cultures from these two cultivars were induced in an embryogenesis establishment medium (CIM) from ovaries obtained from flowers 10-14 days pre-anthesis. The embryogenic lines obtained in this experiment have been stably maintained for more than 2 years, through repeated subcultures on a long-term maintenance medium (LTMM) without loss of embryogenic competence. Somatic embryo regeneration and maturation have been successfully achieved after 30 days of cultivating embryogenic cultures in an embryo regeneration medium (EDMM), supplemented with charcoal and polyethylene glycol. The somatic embryos were successfully germinated in two different media, 'Fredonia' germination medium (FGM) and 'Niagara' germination medium (NGM), and converted into normal looking plants on a conversion medium (CM).
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