Effects of S,S-ethylenediamine disuccinic acid on the phytoextraction efficiency of Solanum nigrum L. and soil quality in Cd-contaminated alkaline wheat soil

Degradable chelating agent–assisted phytoextraction is a promising method for the remediation of Cd-contaminated agricultural soil. However, there are limited studies that have examined the effect of chelating agents on soil solutions and soil quality in alkaline soil. In this study, the effects of S,S-ethylenediamine disuccinic acid (EDDS) on the growth and phytoextraction of Solanum nigrum L. (S. nigrum) were studied using pot experiments. The influence of EDDS on the soil solutions, heavy metal contents, and soil enzyme activities was evaluated. EDDS application increased the height of S. nigrum by 7.25–29.25 cm and increased the biomass of stem and leaf by 4.26–14.95 and 1.14–10.78 g/pot, respectively. The Cd concentrations in the leaves and berries of S. nigrum were 1.21–2.17 and 1.7–9.47 times higher than that of the control, respectively, and the Cd extraction amount in the shoots of S. nigrum increased by 22.78–256.16 μg/pot after EDDS application. The chelation of EDDS on heavy metals reached a peak after 7 days of application, decreased gradually with the degradation of EDDS, and disappeared after 30 days of application. Soil pH, available metals, metal speciation, and soil urease were significantly related to the application time of EDDS. Importantly, EDDS application 45 days before S. nigrum harvest treatments decreased the available metal concentrations and improved soil pH and urease activity. However, when EDDS was applied 15 days before S. nigrum harvest, the available Cd and Pb concentrations significantly increased and caused additional Pb pollution. Considering the chelation and degradation effects, the environmental implication, and the cost of EDDS, the results of this study showed that one application of EDDS was better than two applications, a 45-day application before harvest was preferred to a 15-day application, and application of 1–3 mM EDDS 30–45 days before S. nigrum harvest was the most promising application method for the remediation of Cd-contaminated alkaline soil.