Efficacy of Biosynthesised Silver Nanoparticles From Entomopathogenic Fungi Metarhizium Rileyi Against Spodoptera Litura on Cabbage
2021
Jamunarani, G S | Ramanagoljda, S H | Satyanarayana, C | Mulla, S R | Vinaykumar, M M | Ghande, Anita Rajkumar
There is lot of scope for applications of nanotechnology in agriculture. Nanobased insecticide formulations show less toxicity against non-target organisms due to absence of organic solvents. Hence, in this study silver nanoparticles were biosynthesized from Metarhizium rileyi Farl. and the synthesis confirmed initially by colour change from pale yellow to dark brown after seven days. Further, these were characterized using UV-visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM) and EDAX analysis. The surface plasmon resonance peaks were observed at 311 nm and 315 nm in 1.75 and 2.00 mM concentration, respectively. The particle size ranged from 64.11 to 99.08 nm in 1.75 mM and 64.11 to 128.2 nm in 2.00 mM concentration under SEM. In TEM analysis the particle size ranged from 12.80 to 22.20 nm in 1.75 mM and 11.00 to 19.00 nm in 2.00 mM concentration. The presence of elemental silver was confirmed by signals that ranged from 0 to 10 keV in EDAX analysis. The synthesized nanoparticles were effective in suppressing the Spodoptera litura F. both under laboratory and pot culture experiments. The maximum mortality of80.00 and 53.33% was observed at 1.75 and 2.00 mM under laboratory condition, in the 2nd and 3rd instar larvae, respectively. Whereas, in pot culture the mortality was 73.75 and 78.75% with 1.75- and 2.00-mM concentrations, respectively, as compared to the commercial formulation of M. rileyi and NSKE 4%.
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