Characterizing the production of a wild-type and benomyl-resistant Fusarium lateritium for biocontrol of Eutypa lata on grapevine
2001
McMahan, G. | Yeh, W. | Marshall, M.N. | Olsen, M. | Sananikone, S. | Wu, J.Y. | Block, D.E. | VanderGheynst, J.S.
Benomyl-resistant (BR) and wild-type (WT) strains of Fusarium lateritium were examined for their tolerance to benomyl on potato dextrose agar (PDA) containing benomyl and control of the Eutypa lata in grapevine bioassays. The WT strain grew on PDA containing 1 microgram/ml benomyl at 13, 26 and 29 degrees C. The BR strain grew on PDA containing 10 microgram/ml benomyl at 4 degrees C, on PDA containing 100 microgram/ml benomyl at 29 degrees C, and on PDA containing 1000 microgram/ml benomyl at 13 degrees C and 26 degrees C. The BR strain was also able to colonize grapevine segments and control E. lata in the presence of 1000 microgram/ml benomyl. Both strains were amenable to production via liquid fermentation and both achieved 100% control of E. lata in grapevine bioassays. Neither the duration of fermentation nor incubation temperature during grapevine bioassays influenced the efficacy of either strain against E. lata. The results suggest that application of BR F. lateritium alone or in combination with benomyl may provide good control of E. lata.
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