Determination of formetanate hydrochloride in strawberries
1996
Hu, R. | Petay, V. | Fournier, J.
A method was established for determining formetanate hydrochloride in strawberries by reversed-phase high-performance liquid chromatography (HPLC). Formetanate hydrochloride was extracted from strawberries with acidified organic solvents (acetonitrile:HCl:ethyl acetate), and the extract was purified with a solid-phase extraction (SPE) column followed by a strong cation-exchange (SCX) cartridge, and analyzed by HPLC with UV diode array detection at lambda m of 254 nm. The mobile phase was 0.01 M, pH 8 ammonium phosphate buffer:acetonitrile (70:30). Formetanate was quantitated by the internal standard method. The percent recovery averaged 87.4 +/- 2.2 (0.167 mg kg-1), 89.0 +/- 2.1 (1.67 mg kg-1), and 91.9 +/- 3.2 (16.7 mg kg-1), the limit of determination (LD) was 0.018 mg kg-1, and the limit of quantitation (LQ) was 0.18 mg kg-1.
显示更多 [+] 显示较少 [-]