Label-free, nucleotide-mediated dispersion of magnetic nanoparticles for “non-sandwich type” MRI-based quantification of enzyme
2013
Liang, Guohai | Xiao, Lifu | Chen, Hui | Liu, Qian | Zhang, Song | Li, Fuyou | Kong, Jilie
In the present work, we demonstrate that nucleotide can adsorb efficiently on the surface of carboxylic acid-functionalized nanoparticles and stabilize the particles against aggregation. In the present study we take magnetic nanoparticles (MNPs), manganese oxide nanoparticles (MnO), and upconversion nanophosphors (UCNPs) as models. The result shows that not only MNPs, but also other kinds of nanoparticles that have similar surface properties can be dispersed and stabilized by nucleotides. Interestingly, adenosine bearing different numbers of phosphate groups has distinct stabilizing effect. On the basis of this observation, we developed a magnetic relaxation-based enzyme assay for quantitative analysis of alkaline phosphatase. A detection limit of 0.002U/μL for calf intestine alkaline phosphatase (CIAP) could be obtained, which is lower than the gold nanoparticle-based colorimetric method. In contrast to the conventional magnetic relaxation switches (MRSw), this assay was achieved without covalent modification and separation steps, sandwich type binding was not required as well, which would potentially expand the application of magnetic relaxation-based analysis.
显示更多 [+] 显示较少 [-]