Poly(3-hydroxybutyrate) production using mannitol as a sole carbon source by Burkholderia sp. AIU M5M02 isolated from a marine environment
2018
Yamada, Miwa | Yukita, Ai | Hanazumi, Yuta | Yamahata, Yuki | Moriya, Hiroki | Miyazaki, Masao | Yamashita, Tetsuro | Shimoi, Hitoshi
We isolated Burkholderia sp. AIU M5M02, which accumulates poly(3-hydroxybutyrate) [P(3HB)] homopolymer in a nitrogen-limiting mineral salt medium containing mannitol as a sole carbon source, from a marine environment. When the isolated strain was cultured in a nitrogen-limiting mineral salt medium (pH 5.0) containing 10% (v/v) mannitol as a sole carbon source for 4 days, P(3HB) content and P(3HB) productivity reached 40 weight percent and 1.5 g/l, respectively. Thus, we demonstrated that Burkholderia sp. AIU M5M02 can be utilized for P(3HB) production from mannitol, which is a major sugar in seaweed. Furthermore, the isolate utilized not only mannitol but also gluconic acid, glycerol, starch, and sugars such as fructose, glucose, maltose, sucrose, and xylose to produce P(3HB). Based on these results, we expect to be able to apply Burkholderia sp. AIU M5M02 to the production of P(3HB) from other raw materials with low environmental loads.
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