Oceanisphaera pacifica sp. nov., isolated from the intestine of Trichiurus japonicus
2022
Xue, Jian-Heng | Shi, Li-Fang | Zhang, Bei-Ning | Wu, Wen-Jie | Gao, Yuan | Zhu, Qian | Zhao, Li-Hua
A Gram-stain-negative, strictly aerobic, non-flagellated, oxidase- and catalase-positive, rod-shaped marine bacterium, designated strain DM8ᵀ, was isolated from the intestine of Trichiurus japonicus in Weihai, China. The strain optimally grew at 25–35℃, with 1.0–4.0% (w/v) NaCl and at pH 7.0–8.0. Its colonies were circular, slightly yellow, non-transparent, smooth, and approximately 0.8–1.5 mm in diameter, after being cultured for 48 h on marine agar 2216. Based on the result of phylogenetic analysis of 16S rRNA gene sequence, strain DM8ᵀ had close relationship with Oceanisphaera profunda SM1222ᵀ (96.9%) and the type strain DSM 15406 ᵀ of the type species Oceanisphaera litoralis (94.7%), respectively. Genome sequencing revealed a genome size of 3,109,059 bp and a G + C content of 46.9 mol%. It had Q-8 as the sole respiratory quinone and possessed C₁₆:₀, summed features 3 (C₁₆:₁ω7c/C₁₆:₁ω6c) and summed features 8 (C₁₈:₁ω7c/C₁₈:₁ω6c) as major fatty acids. The major polar lipid profile was composed of phosphatidylglycerol and phosphatidylethanolamine. Based on the phenotypic, chemotaxonomic characterizations, phylogenetic properties and genome analysis, strain DM8ᵀ should represent a novel species of the genus Oceanisphaera, for which the name Oceanisphaera pacifica sp. nov. is proposed. The type strain is DM8ᵀ (= KCTC 82764 ᵀ = MCCC 1K06133ᵀ).
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