Parkia pendula Seed Lectin: Potential Use to Treat Cutaneous Wounds in Healthy and Immunocompromised Mice
2014
Coriolano, Marília Cavalcanti | de Melo, Cristiane Moutinho Lagos | Silva, Flávio de Oliveira | Schirato, Giuliana Viegas | Porto, Camila Souza | dos Santos, Paulo Jorge Parreira | Correia, Maria Tereza dos Santos | Porto, Ana Lúcia Figueiredo | Carneiro-Leão, Ana Maria dos Anjos | Coelho, Luana Cassandra Breitenbach Barroso
Parkia pendula seed lectin was used to treat cutaneous wounds of normal and immunocompromised mice, inducing cicatrization. Methotrexate (0.8 mg/kg/week) was used as immunosuppressive drug. Wounds were produced in the dorsal region (1 cm²) of female albino Swiss mice (Mus musculus), health and immunocompromised. Wounds were daily topically treated with 100 μL of the following solutions: (1) control (NaCl 0.15 M), (2) control Im (0.15 M NaCl), (3) P. pendula seed lectin (100 μg/mL), and (4) P. pendula seed lectin Im (100 μg/mL). Clinical evaluation was performed during 12 days. Biopsies for histopathology analysis and microbiological examinations were carried out in the second, seventh, and 12th days. The presence of edema and hyperemia was observed in all groups during inflammatory period. The first crust was detected from the second day, only in the groups treated with P. pendula seed lectin. Microbiological analysis of wounds from day 0 to day 2 did not show bacterium at P. pendula seed lectin group; however, Staphylococcus sp. was detected every day in the other groups. The lectin markedly induced a total wound closing at P. pendula seed lectin and P. pendula seed lectin Im groups on 11th day of evolution. The present study suggests that P. pendula seed lectin is a biomaterial potential to show pharmacological effect in the repair process of cutaneous wounds.
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