Overestimation of the Abundance of Sulfate-Reducing Bacteria in Human Feces by Quantitative PCR Targeting the Desulfovibrio 16S rRNA Gene
2011
Christophersen, C.T. | Morrison, M. | Conlon, M.A.
The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism.
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书目信息
出版者
The Royal Society of Chemistry
其它主题
Desulfovibrio; Sulfate-reducing bacteria; Gene targeting
语言
英语
注释
Includes references
许可
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类型
Journal Article; Text
2024-02-29
MODS