Development and Validation of a Simple Method for Routine Analysis of Ractopamine Hydrochloride in Raw Material and Feed Additives by HPLC
2009
Freire, Ellen Figueiredo | Borges, Keyller Bastos | Tanimoto, Hélio | Nogueira, Raquel Tassara | Bertolini, Lucimara Cristiane Toso | Gaitani, Cristiane Masetto de
A simple method was optimized and validated for determination of ractopamine hydrochloride (RAC) in raw material and feed additives by HPLC for use in quality control in veterinary industries. The best-optimized conditions were a C8 column (250 x 4.6 mm id, 5.0 μm particle size) at room temperature with acetonitrile-100 mM sodium acetate buffer (pH 5.0; 75 + 25, v/v) mobile phase at a flow rate of 1.0 mL/min and UV detection at 275 nm. With these conditions, the retention time of RAC was around 5.2 min, and standard curves were linear in the concentration range of 160-240 μg/mL (correlation coefficient >or=0.999). Validation parameters, such as selectivity, linearity, limit of detection (ranged from 1.60 to 2.05 μg/mL), limit of quantification (ranged from 4.26 to 6.84 μg/mL), precision (relative standard deviation <or=1.87%), accuracy (ranged from 96.97 to 100.54%), and robustness, gave results within acceptable ranges. Therefore, the developed method can be successfully applied for the routine quality control analysis of raw material and feed additives.
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