R. oryzae Cellulases: A New Approach to Degrading Lignocellulosic Material
2015
Kupski, Larine | Carvalho Silvello, Maria Augusta | Fontes, Milena Ramos Vaz | Lima, Tiago Silva | Treichel, Helen | Badiale Furlong, Eliana
During the fermentative processes with Rhizopus oryzae CCT 7560 and Trichoderma reesei QM 9414, the effects of rice hull and moisture content on the endoglucanase and total cellulase activities were investigated by response surface methodology, employing a central composite rotatable design. The nutrient solution and bioreactor design were also studied. In tray bioreactors maximum cellulase activity was achieved with 82.5% of rice hull and 17.5% of rice bran, with an initial moisture content of 30% for Rhizopus, while 100% of rice hull with an initial moisture content of 50% was used for Trichoderma in Erlenmeyer bioreactors. The results obtained for Rhizopus were 91% higher for endoglucanase when compared with Trichoderma, confirming the potential of the newly isolated R. oryzae CCT 7560 for cellulase production. In addition, this provided an economical and environmentally viable destination for one of the biggest wastes resulting from the rice industry, the rice hull. PRACTICAL APPLICATIONS: Most cellulases related in the literature are obtained from fermentative processes employing genetically modified microorganisms during extensive time to achieve maximum activity. The microorganism studied in this work (Rhizopus oryzae CCT 7560) exhibited rapid enzymatic production indicating its use to obtain digestible carbohydrates from agribusiness waste in a quick and safe way.
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