Two-way effect of pesticides on zeatin riboside content in both rice leaves and roots
2004
Qiu, Z.H. | Wu, J.C. | Dong, B. | Li, D.H. | Gu, H.N.
Cytokinins zeatins including zeatins riboside (ZR) play a vital regulation role in growth, development, physiology and biochemistry of rice plant. The effect of four commonly used pesticides in paddy fields on the ZR contents in rice leaves and roots, was investigated using Enzyme-Linked Immunosorbent Assays (ELISA). Experimental rice plants were grown under hydroponics culture conditions, and subjected to a foliar spray or root treatment with different concentrations of these pesticides. Zeatin riboside content in rice leaves decreased significantly three days after foliar sprays (3 DAS) with 150 and 300 ppm buprofezin, 30 and 60 ppm imidacloprid, 200 ppm jinganmycin, and 480 ppm triazophos. At 7 DAS a significant reduction occurred irrespective of the pesticide concentration. The ZR content in rice roots did not change so dramatically as in rice leaves. At 3 DAS, it was reduced significantly only in the plants subjected to a foliar spray with 100 ppm jinganmycin or significantly increased in the plants sprayed with 480 ppm triazophos, while at 7 DAS, there were no significant differences in ZR content under all circumstances. When subjected to root treatment with these pesticides, rice plants were extremely sensitive to triazophos and even wilted three days after the treatments (3 DART). Root treatment with 150 ppm buprofezin, 100 ppm jinganmycin, 60 ppm imidacloprid, respectively, caused a significant reduction in ZR contents in rice leaves; however, all treatments except with triazophos, did not reduce ZR contents in rice roots significantly. Seven days after foliar sprays with the pesticide mixtures, i.e. triazophos+imidacloprid and triazophos+buprofezin. ZR content significantly reduced in rice leaves but not in roots.
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