Tetrapyrrole Accumulation in in vitro Selected Somaclones of Acifluorfen-Tolerant Solanum ptycanthum Dun
2004
Yu, Chang Yeon | Chun, Se Chul | Lim, Jung Dae | Kim, Seung-Hyun | Kang, Won Hee | Chung, Ill Min
Acifluorfen-tolerant callus lines of Solanum ptycanthum were isolated by stepwise selection. Growth of unselected lines was completely inhibited at 0.5 µM acifluorfen, while some selected lines grew at 8 µM acifluorfen. Twenty-two of 25 acifluorfen-tolerant callus lines regenerated shoots. Acifluorfen-tolerant S. ptycanthum callus lines differed in protoporphyrin IX content ranging from 2.0 to 43.5 nmole per 100 mg protein. As the concentration of acifluorfen increased, the amount of protoporphyrin IX accumulated increased. These results indicated that the possible site of action of acifluorfen was protoporphyrinogen oxidase which might be the molecular target of the herbicide within plant cell.
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