Immunoassay for detection of zearalenone in agricultural commodities
1991
Bennett, G.A.
A direct competitive enzyme-linked immunosorbent assay for the detection of zearalenone in corn, wheat, and feed has been evaluated by 23 collaborators in an international collaborative study. Both visual and spectrophotometric determinations of zearalenone were done on blind duplicates of spiked and naturally contaminated samples of each commodity. Frequency of false negatives was 25% at the target level of 500 ng zearalenone/g commodity, but was only 3.4% at 800 ng zearalenone. No noticeable matrix effect was observed for the samples tested. Coefficients of variation for repeatability were 11.6% and 11.7% for spiked and naturally contaminated samples, respectively. Coefficients of reproducibility were 25.1% and 33.1% for spiked and naturally contaminated samples, respectively. This study demonstrates the reliability of the immunoassay procedure as a screening method for zearalenone at >800 ng/g in corn, wheat, and feed.
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