Antibodies against the calcium-binding protein. Calsequestrin from Streptanthus tortuosus (Brassicaceae)
1989
Chou, M. | Krause, K.H. | Campbell, K.P. | Jensen, K.G. | Sjolund, R.D.
Plant microsomes contain a protein clearly related to a calcium-binding protein, calsequestrin, originally found in the sarcoplastic plasmic reticulum of muscle cells, responsible for the rapid release and uptake of Ca2+ within the cells. The location and role of calsequestrin in plant cells is unknown. To generate monoclonal antibodies specific to plant calsequestrin, mice were immunized with a microsomal fraction from cultured cells of Streptanthus tortuosus (Brassicaceae). Two clones cross-reacted with one protein band with a molecular weight equal to that of calsequestrin (57 kilodaltons) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. This band is able to bind Ca2, and can be recognized by a polyclonal antibody against the canine cardiac muscle calsequestrin. Rabbit skeletal muscle calsequestrin cross-reacted with the plant monoclonal antibodies. The plant monoclonal antibodies generated here are specific to calsequestrin protein.
显示更多 [+] 显示较少 [-]