Optimization of Simultaneous Saccharification and Fermentation Parameters for Sustainable Ethanol Production from Sugarcane Bagasse by Pachysolen tannophilus MTCC 1077
2016
Bhatia, Latika | Johri, Sonia
Pulverized sugarcane bagasse consists of 37 ± 0.29 % cellulose, 28 ± 0.26 % hemicellulose, and 21 ± 0.28 % lignin on dry solid basis. About 11.58 % xylose, 6.39 % glucose, and 4.56 % fructose were liberated in hydrolysate when bagasse was treated with 1 % HNO₃, under steam explosion. Simultaneous saccharification and fermentation of pretreated bagasse by cellulase and Pachysolen tannophilus MTCC 1077 were investigated in the present study. Important process variables for ethanol production from pretreated bagasse were optimized using response surface methodology based on central composite design (CCD) experiments. A three-level CCD experiments with central and axial points was used to develop a statistical model for the optimization of process variables. Data obtained from RSM on ethanol production were further subjected to the analysis of variance, and contour plots were used to study the interactions among three relevant variables (incubation temperature, inoculum concentration, and nutrient factors) of the fermentation process. Maximum ethanol concentration 9.15 g/l was obtained after 72-h incubation with P. tannophilus MTCC 1077 at the optimized process conditions in anaerobic batch fermentation when optimum values for temperature (34 °C), inoculum level (6 %), and fermentation medium (ammonium sulfate, KH₂PO₄, peptone, and yeast extract) were applied.
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