Screening of Sesame Cultivars with Scant Albumins by an Immunological Approach
2018
Kishikawa, Akihiro | Inoue, Maya | Tarutani, Shotaro | Okunishi, Takumi | Ina, Rie | Yoshida, Kazushi | Takada, Naoyuki | Yoshida, Motonobu
In this study a detection method for 2S albumin (14-kDa albumin) among the sesame allergens was developed. Polyclonal antibodies against 14-kDa albumin from sesame seeds (anti 14-kDa albumin antibody) were prepared. The anti 14-kDa albumin antibody absorbed with acetone powder from soybean seeds specifically reacted with sesame albumin, and did not react with albumins from soybean, corn, Arabidopsis. The absorbed anti 14-kDa albumin antibody was used as a probe for screening sesame varieties with scant albumins by indirect enzyme-immunosorbent assay (ELISA). We isolated accession number (Acc. No.) 526 with one-third of albumin amounts compared to standard variety Acc. No. 800. This method will be useful in breeding sesame cultivars with low sesame allergens
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