Cloning and Transcript Analyses of the Chimeric Gene Associated With Cytoplasmic Male Sterility in Cauliflower (Brassica oleracea var. botrytis)
2006
Wang, Chunguo | Chen, Xiaoqiang | Lan, Tianying | Li, Hui | Song, Wenqin
To gain a preliminary understanding of cytoplasmic male sterility (CMS) in cauliflower NKC-A, seven primer pairs were designed to amplify atpa, atp6, atp9, coxI, coxII, orfB and orf224, which have been reported in other CMS systems. The amplification pattern of orfB is different in the CMS cauliflower and its maintainer line NKC-B. Results suggest that orfB only expresses in the CMS line, in both flowers and leaves. Sequence analysis indicated that orfB is highly homologous with orf138, a chimeric gene believed to closely associate with Ogura cytoplasmic male sterility in radish. Full-length sequence was obtained to elucidate the molecular basis of orfB. The sequence includes three open reading frames, trnfM, orf138 and orf158. Study of transcriptional patterns and RNA editing of this sequence revealed that orf138 and orf158 co-transcribe, similar to orf138 in Ogura CMS radish. Based on this, we concluded that the molecular basis of cytoplasmic male sterility in cauliflower NKC-A may be similar to that of the Ogura CMS in radish. However, the nuclear backgrounds and RNA editing of corresponding sequences in radish and cauliflower are different. The results of this study also provide important information for breeders selecting new CMS lines in cauliflower. A specific molecular marker was developed according to the CMS specific fragment in NKC-A.
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