Identification of 36 kDa phosphoprotein in fibrous sheath of hamster spermatozoa
2004
Fujinoki, M. | Kawamura, T. | Toda, T. | Ohtake, H. | Ishimoda-Takagi, T. | Shimizu, N. | Yamaoka, S. | Okuno, M.
In our previous studies (Fujinoki et al., 2001, 2003), we reported that two types of 36 kDa proteins, designated 36K-A protein and 36K-B protein, obtained from hamster sperm flagella, are associated with motility activation and phosphorylated in a cAMP-dependent manner at serine residues. In the present experiments, we focused on the hamster (Mesocricetus auratus) 36K-A protein, which was analyzed by peptide mass finger printing and amino acid sequencing. The results suggest that 36K-A protein is a pyruvate dehydrogenase E1 component beta subunit lacking the N-terminal 30 amino acids. Moreover, our results suggest that 36 K-A protein is localized in the fibrous sheath of the principal piece of hamster spermatazoa.
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